A Reverse phase high performance liquid chromatography method developed for the simultaneous estimation of ambroxol HCl and doxofylline in tablet formulation. The experiment was carried out on C2695 waters HPLC by using empower software. The separation was carried out by X-Terra C18 (150 mm x 4.6, 5μm) column and Ammonium acetate buffer (0.01M, 6.4PH): ACN (60:40) used as a mobile phase in isocratic technique, at a flow rate of 1 ml/min. The detection was carried out at 257nm. The retention time of Ambroxol HCl and Doxofylline were found to be 2.5 and 5.7 min, respectively. The method has been validated as for ICH guidelines for Accuracy, Precision, Linearity and robustness and the results obtained were within the limits. The Linearity for Ambroxol HCl and Doxofylline were found to be in the range of 0.75-4.5 ppm and 10-60 ppm, respectively. The mean recoveries obtained for Ambroxol HCl and Doxofylline were found to be 99.5% and 99.9%, respectively. The Developed method was found to be accurate, precise, selective for simultaneous estimation of Ambroxol HCl and Doxofylline in tablets.
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